PRP會刺激並促進人體膠原蛋白的合成與活性
PRP會刺激並促進人體膠原蛋白的合成與活性
PRP能刺激脯胺酸肽酶活性，類似於膠原蛋白的生物合成，膠原蛋白生物合成和脯胺酸肽酶活性受β1-整聯蛋白受體信號傳導的調節。將PRP加入纖維母細胞24 小時會呈現劑量依賴性地增加 β1-整聯蛋白受體的表達，經研究的血液部分都增加了 FAK 的表達以及磷酸化 MAP 激酶的表達，可知 PRP 是這些特定激酶表達的最有效刺激物。
PRP科技新知- PRP會刺激並促進人體膠原蛋白的合成與活性
研究目的:
評估富含血小板血漿 (PRP)、離心獲得的 PRP 上清液 (SPRP) 和 Ca(2+) 溶液處理PRP 後獲得的活化 PRP 上清液 (SActi-PRP) 對膠原蛋白的生物合成、脯胺酸肽酶(prolidase)活性和人體皮膚纖維母細胞中的β1-整聯蛋白(integrin)信號傳導的影響。
研究結果:
與對照組相比，將 5% PRP 加入纖維母細胞(fibroblasts) 24 小時，有助於膠原蛋白的生物合成增加約 5 倍；相對的，使用 5% 的 SPRP 或 SActi-PRP 加入纖維母細胞(fibroblasts)，膠原蛋白生物合成增加約 3 倍。 PRP、SPRP 和 SActi-PRP 皆能刺激脯胺酸肽酶活性，類似於膠原蛋白的生物合成。膠原蛋白生物合成和脯胺酸肽酶活性受β1-整聯蛋白受體信號傳導的調節。將PRP加入纖維母細胞24 小時會呈現劑量依賴性地增加 β1-整聯蛋白受體的表達，而 SActi-PRP 將該過程增加的程度要低得多。 SPRP 對β1-整聯蛋白受體的表達沒有影響。所有研究的血液部分都增加了 FAK 的表達以及磷酸化 MAP 激酶的表達。然而，發現 PRP 是這些特定激酶表達的最有效刺激物。
結論:
PRP 中所含的多種成分，包括生長因子、粘附分子和脯胺酸肽酶，都能夠刺激並促進人體膠原蛋白的合成與活性。
原文摘要:
The study was conducted to evaluate the effects of platelet-rich plasma (PRP), supernatant of PRP (SPRP) obtained by centrifugation, and supernatant of activated PRP (SActi-PRP) obtained by Ca(2+) solution-treated PRP on collagen biosynthesis, prolidase activity, and beta1-integrin signaling in cultured human skin fibroblasts. Incubation of fibroblasts with 5% PRP for 24 h contributed to ~5-fold increase in collagen biosynthesis compared to the control. In the cells treated with 5% of SPRP or SActi-PRP, collagen biosynthesis showed a 3-fold increase of the control. PRP, SPRP, and SActi-PRP stimulated prolidase activity similar to collagen biosynthesis. Collagen biosynthesis and prolidase activity are regulated by beta1-integrin receptor signaling. Incubation of fibroblasts with PRP for 24 h contributed to a dose-dependent increase in the expression of beta1-integrin receptor, while SActi-PRP increased the process to a much lower extent. SPRP had no effect on the beta1-integrin receptor expression. All the studied fractions of blood increased the expression of FAK as well as the expression of phosphorylated MAP-kinases. However, PRP was found to be the most effective stimulator of expression of these particular kinases. These studies suggest that a complex of factors, including growth factors, adhesion molecules, and prolidase contained in PRP, all evoke growth and collagen-promoting activities in human dermal fibroblasts.
文章出處:
Guszczyn T, Surazynski A, Zareba I, Rysiak E, Popko J, Palka J: Differential effect of platelet-rich plasma fractions on beta1-integrin signaling, collagen biosynthesis, and prolidase activity in human skin fibroblasts. Drug Des Devel Ther 2017, 11:1849-1857.
研究目的:
評估富含血小板血漿 (PRP)、離心獲得的 PRP 上清液 (SPRP) 和 Ca(2+) 溶液處理PRP 後獲得的活化 PRP 上清液 (SActi-PRP) 對膠原蛋白的生物合成、脯胺酸肽酶(prolidase)活性和人體皮膚纖維母細胞中的β1-整聯蛋白(integrin)信號傳導的影響。
研究結果:
與對照組相比，將 5% PRP 加入纖維母細胞(fibroblasts) 24 小時，有助於膠原蛋白的生物合成增加約 5 倍；相對的，使用 5% 的 SPRP 或 SActi-PRP 加入纖維母細胞(fibroblasts)，膠原蛋白生物合成增加約 3 倍。 PRP、SPRP 和 SActi-PRP 皆能刺激脯胺酸肽酶活性，類似於膠原蛋白的生物合成。膠原蛋白生物合成和脯胺酸肽酶活性受β1-整聯蛋白受體信號傳導的調節。將PRP加入纖維母細胞24 小時會呈現劑量依賴性地增加 β1-整聯蛋白受體的表達，而 SActi-PRP 將該過程增加的程度要低得多。 SPRP 對β1-整聯蛋白受體的表達沒有影響。所有研究的血液部分都增加了 FAK 的表達以及磷酸化 MAP 激酶的表達。然而，發現 PRP 是這些特定激酶表達的最有效刺激物。
結論:
PRP 中所含的多種成分，包括生長因子、粘附分子和脯胺酸肽酶，都能夠刺激並促進人體膠原蛋白的合成與活性。
原文摘要:
The study was conducted to evaluate the effects of platelet-rich plasma (PRP), supernatant of PRP (SPRP) obtained by centrifugation, and supernatant of activated PRP (SActi-PRP) obtained by Ca(2+) solution-treated PRP on collagen biosynthesis, prolidase activity, and beta1-integrin signaling in cultured human skin fibroblasts. Incubation of fibroblasts with 5% PRP for 24 h contributed to ~5-fold increase in collagen biosynthesis compared to the control. In the cells treated with 5% of SPRP or SActi-PRP, collagen biosynthesis showed a 3-fold increase of the control. PRP, SPRP, and SActi-PRP stimulated prolidase activity similar to collagen biosynthesis. Collagen biosynthesis and prolidase activity are regulated by beta1-integrin receptor signaling. Incubation of fibroblasts with PRP for 24 h contributed to a dose-dependent increase in the expression of beta1-integrin receptor, while SActi-PRP increased the process to a much lower extent. SPRP had no effect on the beta1-integrin receptor expression. All the studied fractions of blood increased the expression of FAK as well as the expression of phosphorylated MAP-kinases. However, PRP was found to be the most effective stimulator of expression of these particular kinases. These studies suggest that a complex of factors, including growth factors, adhesion molecules, and prolidase contained in PRP, all evoke growth and collagen-promoting activities in human dermal fibroblasts.
文章出處:
Guszczyn T, Surazynski A, Zareba I, Rysiak E, Popko J, Palka J: Differential effect of platelet-rich plasma fractions on beta1-integrin signaling, collagen biosynthesis, and prolidase activity in human skin fibroblasts. Drug Des Devel Ther 2017, 11:1849-1857.